Proprietary Immunization Platform to Break Immune Tolerance and Raise Antibodies Against Challenging Targets
Challenging targets include:
- Low Immunogenicity
- Extracellular Loop
- Conformational Epitopes
Three Key Components Designed to Mimic Natural Epitopes, and to Initiate and Focus the Adaptive Immune Response Against Targets
1. Immunogen Design
- Format: DNA, peptide, protein, etc.
- Molecular configuration: targetsequence, immune-engaging element,and structural constraint scaffolding
- Immune focusing
- Elements to prolong immunogenexpression in vivo
2. Stand-Alone Immune-Engaging Elements
- Attract APCs, facilitate B cellactivation, survival, and maturation
- Prolong immunogen release in vivo
3. Immunization Regimen
- Heterologous Prime + Boost strategy forimmune focusing and B cell maturationto generate high-affinity antibodies
- Proprietary host selection, formulation,immunization route, dose, and schedule
Rational Immunogen Design & Antigen Display Platforms With Novel Immune-Engaging Elements
Genetic Prime – Protein Boost Immunization
Case Study: Conigen’s Immune-Engaging System Enhanced Antibody Responses in Mice
Target-Specific Antibody Response Rates
Target-Specific Antibody Titers
Apply a fit-for-target approach to each immunization campaign
- Analyze and characterize each target’ssequence features
- Design unique immunization strategies foreach target
Examples: Immune Mouse Sera Specific Binding to GPCR Target Expressing Live Cells by Flow Cytometry
Binding to GPR19 on Live Cells
Binding to GPR56 on Live Cells
Example Target-Specific Antibodies Generated Against GPCR Targets in Mice Sera
CXCR5 | GPRC5D | GPR19 | NK1R | PAR1 | DRD2 | NK3R | LPAR1 | CNR2 | SSTR2 | GLP1R | LGR4 | LGR5 | GPR56
Other Targets
Claudin 18.2 | Nectin-4 | CFTR | CD3e/d
Examples: GPCR –Specific Monoclonal Antibodies Bind to Target Expressing Live Cells by Flow Cytometry
DRD2 / Dopamine Receptor D2
mAb binding DRD2 expressing live cells
PAR1 / Proteinase-Activated Receptor
mAb binding PAR1 expressing live cells
Monoclonal Antibody Discovery Services
Talk to us about your custom antibody manufacturing.