testMW: Molecular Weight marker reduced condition NR: CD117 dimer under non-reduced condition
testImmobilized mouse CD117-His-Avi protein dimer (CSP-25193-03) at 2 μg/mL (100 μL/well) can bind anti-mouse CD117 monoclonal antibody with half maximal effective concentration (EC50) range of 3-12.1 ng/mL (QC tested).
testImmobilized mouse stem cell factor (SCF) at 2 μg/mL (100 μL/well) can bind mouse CD117-His-Avi protein dimer (CSP-25193-03) with half maximal effective concentration (EC50) range of 54.7-218.6 ng/mL (QC tested).

Bioactive, Mouse CD117 Protein Dimer, His-Avi Tag

Tumor Associated Antigen

Product Code: CSP-25193-03
Expression Host: HEK293T
Verified Applications: ELISA for CD117-specific antibody and stem cell factor (SCF) ligand protein binding assays. SPR for CD117 and stem SCF ligand protein binding assays
Suggested Applications: BLI for CD117-specific antibody and SCF protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: S25-P527

For Research Use Only (RUO)

Add to Quote

Ready to buy now?
Email us at [email protected] and we’ll help you place your order directly.

Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Mouse cluster of differentiation 117 (CD117), also known as KIT or c-Kit, is a member of the type III receptor tyrosine kinase family. The recombinant mouse CD117 protein dimer (CSP-25193-03) is a cis-homodimer (cis-dimer) and contains a CD117 extracellular domain (UniProt# P05532, amino acids Ser25-Pro527) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse CD117 protein dimer is bioactive and can bind to stem cell factor (SCF). It also binds CD117-specific antibodies. This CD117 protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD117
UniProt #: AA: P05532
Predicted Molecular Weight: 132 kDa
SDS PAGE Molecular Weight: The migration range of the protein dimer with glycosylation under non-reducing condition is >190 kDa on SDS PAGE.
Protein Construct: CD117 protein dimer contains a CD117 extracellular domainfused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.

Background and Alternate Names

Mouse cluster of differentiation 117 (CD117), is a member of the type III receptor tyrosine kinase family. CD117 is also known as KIT, C-Kit, mast/stem cell growth factor receptor (SCFR), KIT proto-oncogene receptor tyrosine kinase, and MASTC. CD117 contains an extracellular domain with five immunoglobulin-like loops, a transmembrane domain, a juxtamembrane domain, and an intracellular domain. CD117 is a Type 1 transmembrane protein, expressed on hematopoietic stem cells, mast cells, melanocytes, germ cells, and interstitial cells of Cajal. CD117 exists as a monomer under normal physical conditions. Upon binding to its natural ligand, stem cell factor (SCF), homodimerization occurs between two CD117 monomers; this homodimerization is essential for its activation. However, oncogenic mutations can cause ligand-independent pathological dimerization and constitutive activation. CD117 is frequently overexpressed or dysregulated in human cancers, including gastrointestinal stromal tumors, acute myeloid leukemia, melanoma, and small cell lung cancer. CD117 is a promising drug target, especially in precision oncology and regenerative medicine. Understanding CD117 dimerization and its activation is crucial for developing targeted therapeutics.

Alternate Names: KIT, C-Kit, cluster of differentiation 117, CD117, PBT, mast/stem cell growth factor receptor, SCFR, KIT proto-oncogene receptor tyrosine kinase, MASTC