Human interleukin 15 receptor alpha subunit (IL-15Rα) is a transmembrane cytokine receptor. The recombinant IL-15Rα dimer protein (CSP-24064) is a cis-homodimer (cis-dimer) and contains an IL-15Rα extracellular domain (UniProt# Q13261, amino acids Ile31-Thr205) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human IL-15Rα dimer protein is bioactive and can bind to interleukin 15 (IL-15). It also binds IL-15Rα -specific antibodies. This IL-15Rα dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL-15Rα
UniProt #: AA: Q13261
Predicted Molecular Weight: 57 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is 120-190 kDa on SDS PAGE.
Protein Construct: IL-15Rα dimer protein contains an IL-15Rα extracellular domainfused with a dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human interleukin 15 receptor alpha subunit (IL-15Rα) is a transmembrane cytokine receptor. IL-15Rα is also known as IL-15Ra, IL15Ra, cluster of differentiation 215 (CD215), and interleukin 15 receptor subunit alpha. IL-15Rα contains an extracellular domain with a single sushi domain (short consensus repeat or complement control protein repeat), which is essential for interleukin 15 (IL-15) binding and IL-15Rα function, a linker/hinge region, and a membrane-proximal proline-threonine-rich region followed by a transmembrane domain, and cytoplasmic domain. IL-15Rα can homodimerize as well as heterodimerize with IL-2Rβ/CD122 and IL-2Rγ/CD132. IL-15Rα specifically binds IL-15 with very high affinity and is capable of binding IL-15 independently of other subunits. IL-15 stimulations of NK cells and T-cells has been shown to sustain long-lasting antitumor immunity, making IL-15Rα an attractive therapeutic target for immunotherapies.
MW: Molecular Weight marker reduced condition
NR: IL-15Rα dimer under non-reduced condition
Immobilized human IL-15Rα dimer protein
His-Avi tag (Cat. No. CSP-24064) at 2 μg/mL (100 μL/well) can bind anti-human IL-15Rα monoclonal antibody