MW: Molecular Weight marker reduced condition NR: Nectin-1 dimer under non-reduced condition

Immobilized human Nectin-1 protein dimer

His-Avi tag (Cat. No. CSP-25195-03) at 2 μg/mL (100 μL/well) can bind anti-human Nectin-1 monoclonal antibody with half maximal effective concentration (EC50) range of 5.5-21.8 ng/mL (QC tested).

Immobilized human Nectin-4 protein dimer

His tag (Cat. No. CSP-24016) at 2 μg/mL (100 μL/well) can bind human Nectin-1 protein dimer

Bioactive, Human Nectin-1 / CD111 Protein Dimer, His-Avi Tag

Tumor Associated Antigen

Product Code: CSP-25195-03

Expression Host: HEK293T

Verified Applications: ELISA for Nectin-1-specific antibody binding assays and ELISA and SPR for Nectin-3 and Nectin-4 ligand protein binding assays.

Suggested Applications: SPR & BLI for Nectin-1-specific antibody binding assays and BLI for Nectin-3 and Nectin-4 protein binding assays. Animal immunization, RUO.

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Human Nectin-1 is a type 1 integral membrane glycoprotein that can form a homo-cis dimer on the cell membrane. The recombinant Nectin-1 dimer protein (CSP-25195-03) is a cis-homodimer (cis-dimer) and contains a Nectin-1 extracellular domain (UniProt# Q15223, amino acids Gln31-Ala355) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Nectin-1 protein dimer is bioactive and can bind to Nectin-3 and Nectin-4. It also binds Nectin-1-specific antibodies. This Nectin-1 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.

Protein Name: Nectin-1

UniProt #: 1: Q15223

Predicted Molecular Weight: 92 kDa

SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 120 and 190 kDa on SDS PAGE.

Protein Construct: Nectin-1 protein dimer contains a Nectin-1 extracellular domainfused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.

Background

Human Nectin-1 is a type 1 integral membrane glycoprotein that can form a homo-cis dimer on the cell membrane. Nectin-1 is also known as Poliovirus receptor-related 1 (PVRL1), cluster of differentiation 111 (CD111), and herpesvirus entry mediator C (HVEC). Nectin-1 contains an extracellular domain with three immunoglobulin-like (Ig-like) domains. Nectin-1 interacts with both Nectin-4 and Nectin-3. Nectin-1 and Nectin-3 are expressed in neuronal tissue and unlike many other cellular adhesion molecules, they do not distribute evenly. Nectin-1 is primarily found on the axonal side and Nectin-3 is primarily found on the dendritic side. The interaction of Nectin-1 with Nectin-3 has been found to be essential for proper morphogenesis of the eye. Nectin-1 also functions as the entry receptor for Herpes Simplex Viruses 1 and 2 through interaction with glycoprotein D on the viral envelope. Therefore, a recombinant protein mimicking the Nectin-1 dimer conformation can be crucial for therapeutic discovery.

Bioactive, Human Nectin-1 / CD111 Protein Dimer, His-Avi Tag

Tumor Associated Antigen

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