His Tag (CSP-25185-01) at 2 μg/mL (100 μL/well) can bind mouse CD80 protein dimer
Fc Tag (Cat. No. CSP-25187-04)
Bioactive, Mouse CTLA-4 Protein Dimer, His Tag
Immune checkpoint
Product Code: CSP-25185-01
Expression Host: HEK293T
Verified Applications: SPR for CTLA-4-specific antibody and CD80 ligand protein binding assays. ELISA for CTLA-4 and CD80 ligand protein binding assays.
Suggested Applications: BLI for CTLA-4-specific antibody and CD80 protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
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Cytotoxic T-lymphocyte associated protein 4 (CTLA-4), is also known as CD152 (cluster of differentiation 152), CTLA4, ALPS5, CD, CD152, CELIAC3, GRD4, GSE, and IDDM12. CTLA-4 is a protein dimer (CSP- 25185-01) and contains a CTLA-4 extracellular domain (UniProt# P09793, amino acids Glu36-Asp161) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The CTLA-4 protein dimer is bioactive and can enhance the binding to CD80. It also binds CTLA-4-specific antibodies. This CTLA-4 protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CTLA-4
UniProt #: AA: P09793
Predicted Molecular Weight: 44 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is 60-85 kDa on SDS PAGE.
Protein Construct: CTLA-4 protein dimer contains a CTLA-4 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Cytotoxic T-lymphocyte associated protein 4 (CTLA-4), also known as CD152 (cluster of differentiation 152), CTLA4, ALPS5, CD, CELIAC3, GRD4, GSE, and IDDM12 is a member of the immunoglobulin superfamily. CTLA-4 contains extracellular immunoglobulin-like (Ig-like) domains (an Ig-V-like and an Ig-C-like domain), a transmembrane domain, and a cytoplasmic tail. As an immune checkpoint CTLA-4 binds both CD80 (Cluster of differentiation 80) and CD86 (Cluster of differentiation 86) to transmit an inhibitory signal with T cells, competing with CD28 (Cluster of differentiation 28) which transmits a stimulatory signal. It is often overexpressed in human malignancies caused by immunosurveillance, making the inhibition of immune checkpoint proteins like CTLA-4 an emerging strategy in cancer therapy. CTLA-4 gene variants have been associated with Type 1 diabetes, Graves’ disease, Hashimoto’s thyroiditis, celiac disease, and other autoimmune diseases. While structurally and functionally similar to human CTLA-4, mouse CTLA-4 is a species-specific tool essential for preclinical studies, basic research and translational research in cancer immunotherapy.
MW: Molecular Weight marker reduced condition
NR: CTLA-4 dimer under non-reduced condition
Immobilized mouse CTLA-4 protein dimer
His Tag (CSP-25185-01) at 2 μg/mL (100 μL/well) can bind mouse CD80 protein dimer
Fc Tag (Cat. No. CSP-25187-04)