TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 and VSTM3. The recombinant TIGIT protein dimer (CSP-25182-04) is a cis-homodimer (cis-dimer) and contains a TIGIT extracellular domain (UniProt# P86176, amino acids Thr29-Gly148) fused with a proprietary cis-dimer motif followed by a His-tag at the C-terminus. This protein is expressed in HEK293T cells. The TIGIT-Fc protein dimer is bioactive and can bind to CD155 and TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.
Protein Name: TIGIT
UniProt #: AA: P86176
Predicted Molecular Weight: 79 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reduced condition is between 120 and 190 kDa on SDS PAGE.
Protein Construct: TIGIT protein dimer contains a TIGIT extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9) and VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a Type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT is a pivotal immune checkpoint receptor that fine-tunes T-cell and NK-cell responses. Its dual role in promoting tolerance (via Tregs) and suppressing antitumor immunity makes it a compelling therapeutic target. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT. While structurally and functionally similar to human TIGIT, mouse TIGIT is a species-specific tool essential for translational research in cancer immunotherapy.
MW: Molecular Weight marker reduced condition
NR: TIGIT dimer under non-reduced condition
Immobilized mouse CD155 protein dimer
His Tag (Cat. No. CSP-25184-01) at 2 μg/mL (100 μL/well) can bind mouse TIGIT protein dimer