MW: Molecular Weight marker reduced condition NR: TIGIT dimer under non-reduced condition

Immobilized mouse TIGIT protein dimer

FLAG-His Tag (Cat. No. CSP-25182-02) at 2 μg/mL (100 μL/well) can bind anti-mouse TIGIT polyclonal antibody with half maximal effective concentration (EC50) range of 11.4-45.6 ng/mL (QC tested).

Immobilized mouse CD155 protein dimer

Bioactive, Mouse TIGIT Protein Dimer, FLAG-His Tag

Immune checkpoint

Product Code: CSP-25182-02

Expression Host: HEK293T

Verified Applications: ELISA for TIGIT-specific antibody binding assays and ELISA and SPR for CD155 ligand protein binding assays.

Suggested Applications: SPR and BLI for TIGIT-specific antibody binding assays and BLI for CD155 protein binding assays. Animal immunization, RUO.

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Mouse TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 and VSTM3. The recombinant TIGIT dimer protein (CSP-25182-02) is a cis-homodimer (cis-dimer) and contains a TIGIT extracellular domain (UniProt# P86176, amino acids Thr29-Gly148) fused with a proprietary dimer motif followed by a tandem FLAG-His tag at the C-terminus. This protein is expressed in HEK293T cells. The TIGIT-Fc dimer protein is bioactive and can bind to CD155 and TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.

Protein Name: TIGIT-Flag-His

UniProt #: 1: P86176

Predicted Molecular Weight: 45 kDa

SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 60 and 85 kDa on SDS PAGE.

Protein Construct: Mouse TIGIT dimer protein contains a TIGIT extracellular domainfused with a proprietary cis-dimer motif followed by a tandem FLAG-His tag at the C-terminus.

Background

TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9) and VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a Type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT is a pivotal immune checkpoint receptor that fine-tunes T-cell and NK-cell responses. Its dual role in promoting tolerance (via Tregs) and suppressing antitumor immunity makes it a compelling therapeutic target. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT. While structurally and functionally similar to human TIGIT, mouse TIGIT is a species-specific tool essential for translational research in cancer immunotherapy.

Bioactive, Mouse TIGIT Protein Dimer, FLAG-His Tag

Immune checkpoint

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