Cluster of Differentiation 38 (CD38), also known as cyclic ADP ribose hydrolase, is a glycoprotein, forms dimers on the cell surface, is found mainly on plasma B cells and natural killer cells, and is often overexpressed in various cancer cells. The recombinant CD38 protein dimer (CSP-24096-03) is a cis-homodimer (cis-dimer) and contains the CD38 extracellular domain (UniProt# P28907, amino acids Val43-Ile300) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CD38 dimer protein binds CD38-specific antibodies. This CD38 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CD38
UniProt #: 1: P28907
Predicted Molecular Weight: 80 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing conditions is between 120 and 190 kDa on SDS PAGE.
Protein Construct: CD38 protein dimer contains the CD38 extracellular domainfused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human Cluster of Differentiation 38 (CD38), also known as cyclic ADP ribose hydrolase, is a glycoprotein found mainly on plasma B cells and natural killer cells. CD38 naturally forms homodimers on the cell surface and functions as an ectoenzyme and as a receptor. CD38 serves as a major regulator of NAD+ levels using its extracellular domain to catalyze the synthesis of ADP ribose (ADPR) and cyclic ADP-ribose (cADPR) from NAD+. CD38 interacts with CD31 on the surface of T cells to activate the production of a variety of cytokines. CD38 is often overexpressed in cancers including multiple myeloma and NK-T cell lymphomas, contributing to cancer cell proliferation, migration, and survival. CD38 expressed on immune cells and airway smooth muscle cells also plays an important role in asthma by influencing inflammation and airway hyper-responsiveness. CD38 is an emerging therapeutic target for several cancers as well as asthma.
MW: Molecular Weight marker reduced condition
NR: CD38 dimer under non-reduced condition
Immobilized human CD38 protein dimer