Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a Type 1 transmembrane protein part of the ROR family of cell surface receptors. ROR1 can form dimers on the cell surface. The recombinant ROR1 dimer protein (CSP-25287) is a cis-homodimer (cis-dimer) and contains the ROR1 extracellular domain (UniProt# Q01973, amino acids Gln30-Tyr406) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human ROR1 dimer protein can bind to ROR1-specific antibodies. This ROR1 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: ROR1
UniProt #: Q01973
Predicted Molecular Weight: 101 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reduced condition is 120-190 kDa on SDS PAGE.
Protein Construct: ROR1 dimer protein contains the ROR1 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is a tyrosine-protein kinase transmembrane receptor, also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1). ROR1 is a Type 1 transmembrane protein part of the ROR family of cell surface receptors. ROR1 contains an extracellular domain with an immunoglobulin-like (Ig-like) domain at the amino-terminus followed by a Frizzled domain (FZD) and Kringle domain (KRD). Like other receptor tyrosine kinases (RTKs), ROR1 can also form dimers on the cell surface. The cysteine-rich FZD mediates the interaction of ROR1 with its ligand, Wnt5a. Generally, ROR1 is only highly expressed and detectable in embryonic tissue during early embryonic development where it modulates neurite growth in the central nervous system and its expression decreases significantly in adult tissues. However, ROR1 is overexpressed on the surface of cancer cells in several types of cancers, including B-cell chronic lymphocytic leukemia (B-CLL), ovarian cancer, breast cancer, and some lung and pancreatic cancers. This explicit expression pattern makes it an excellent cancer marker and an emerging target in cancer therapy.
MW: Molecular Weight marker reduced condition
NR: ROR1 dimer under non-reduced condition
Immobilized human ROR1 protein dimer