Human sialic acid-binding Ig-like lectin 6 (Siglec-6) is a Type I transmembrane protein and a member of the Siglec family and immunoglobulin superfamily. The recombinant Siglec-6 protein dimer (CSP-25203-01) is a cis-homodimer (cis-dimer) and contains a Siglec-6 extracellular domain (UniProt# O43699, amino acids Gln27-Val347) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Siglec-6 dimer protein can potently bind Siglec-6-specific antibodies. This Siglec-6 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: Siglec 6
UniProt #: AA: O43699
Predicted Molecular Weight: 87 kDa
SDS PAGE Molecular Weight: The migration range of the protein dimer with glycosylation under non-reducing condition is 120 to 190 kDa on SDS PAGE.
Protein Construct: Siglec-6 protein dimer contains a Siglec-6 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Human sialic acid-binding Ig-like lectin 6 (Siglec-6) is a Type I transmembrane protein, a member of the Siglec family, part of the immunoglobulin superfamily, and a multifunctional immune regulator. Siglec-6 is also known as Cluster of Differentiation 327 (CD327), CD33-like (CD33L), CD33L1, CD33L2, and CDW327. Siglec-6 contains an extracellular domain with an N-terminal V-type immunoglobulin domain (Ig domain) and two C2-type Ig domains followed by a transmembrane domain and cytoplasmic signaling domain consisting of the immunoreceptor tyrosine-based inhibitory motif (ITIM). Siglec-6 is expressed on trophoblasts and highly expressed on mast cells from a variety of tissues. It binds sialylated glycans on cell surfaces or secreted proteins, modulating cell-cell interactions and immune responses. The presence of Siglec-6 on various kinds of leukemias has made it an emerging target for cancer therapeutics.
MW: Molecular Weight marker reduced condition
NR: Siglec 6 dimer under non-reduced condition
Immobilized human Siglec 6 protein dimer