Mouse colony stimulating factor 2 receptor subunit alpha (CSF2RA) is a Type 1 transmembrane protein and a glycoprotein. The recombinant CSF2RA dimer protein (CSP-25179-03) is a cis-homodimer (cis-dimer) and contains a CSF2RA extracellular domain (UniProt# Q00941, amino acids Leu30-Pro327) fused with a dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse CSF2RA dimer protein is bioactive and can bind CSF2RA-specific antibodies. This CSF2RA dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CSF2R
UniProt #: 1: Q00941
Predicted Molecular Weight: 84 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing conditions is between 120 and 190 kDa on SDS PAGE.
Protein Construct: Recombinant mouse CSF2RA dimer proteinis bioactive.
Background
Colony stimulating factor 2 receptor subunit alpha (CSF2RA) is a Type 1 transmembrane glycoprotein. CSF2RA is also known as granulocyte-macrophage colony-stimulating factor receptor alpha (GMCSFR-alpha), GMRα subunit, and cluster of differentiation 116 (CD116). The CSF2RA extracellular domain contains two fibronectin type III (FN III) domains with a highly conserved WSXWS motif is present near the C-terminus of the second domain. CSF2RA is expressed on (cell types). CSF2RA is primarily located on neutrophils, eosinophils and monocytes/macrophages and is a receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CSF2RA can form a heterodimer with CSF2RB and these heterodimers can form several multimers including a dodecamer. It has been found that CSF2RA specifically has tumor suppression characteristics that have made stimulating CSF2RA expression an emerging strategy in cancer therapy. While structurally and functionally similar to human CSF2RA homodimer, mouse CSF2RA homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.
MW: Molecular Weight marker reduced condition
NR: CSF2Rα dimer under non-reduced condition
Immobilized mouse CSF2Rα protein dimer