Interleukin-6 receptor subunit beta (IL-6Rb) is a transmembrane protein and a member of the class of tall cytokine receptors. The recombinant IL-6Rb protein dimer (CSP-25159-01) is a cis-homodimer (cis-dimer) and contains an IL-6Rb extracellular domain (UniProt# Q00560, amino acids Gln23-Glu617) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse IL-6Rb protein dimer is bioactive and can bind to interleukin-6 (IL-6). It also binds IL-6Rb-specific antibodies. This IL-6Rb dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL-6Rb
UniProt #: AA: Q00560
Predicted Molecular Weight: 149 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is >190 kDa on SDS PAGE.
Protein Construct: Mouse IL-6Rb protein dimer contains an IL-6Rb extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Interleukin-6 receptor subunit beta (IL-6Rb) is a transmembrane protein and a member of the class of tall cytokine receptors. IL-6Rb is also known as Interleukin 6 Cytokine Family Signal Transducer (IL6ST), Cluster of Differentiation 130 (CD130), CDW130, and glycoprotein 130 (gp130). IL-6Rb serves as a shared signal transducing subunit of the receptor complexes for mouse cytokines, including interleukin-6 (IL-6), that mediate highly diverse biological processes. IL-6Rb can form homodimers and heterodimers with other cytokine receptors (i.e., IL-6 receptor alpha (IL-6Ra)) in response to cytokine binding. The homodimerization or heterodimerization of IL-6Rb is key to initiating intracellular signaling pathways. The extracellular domain of IL-6Rb includes an N-terminal immunoglobulin-like (Ig-like) domain (D1), a cytokine-binding homology region (CHR, D2D3), and three membrane-proximal fibronectin type III domains (FNIII, D4 to D6) followed by a transmembrane domain and cytoplasmic domain. It has been found that dysregulation of IL-6Rb expression and signaling mediates progression for multiple types of cancer and autoimmune diseases. Mouse IL-6Rb, the murine homolog of human IL-6Rb with conserved structural domains, is a species-specific tool essential for basic research, translational research and preclinical studies.
