CD80 (B7-1) Proteins

CD80 (B7-1) is a type I transmembrane glycoprotein in the immunoglobulin superfamily and a key member of the B7 family of immune co-stimulatory ligands. It is primarily expressed on antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells, where it regulates T cell responses through binding to CD28 (stimulatory) and CTLA-4 (inhibitory). CD80 is widely studied in immune activation, immune checkpoint biology, autoimmunity, and oncology. Conigen provides recombinant CD80 proteins designed to support receptor/ligand interaction, antibody  binding studies, assay development, and antibody discovery.

Human CTLA-4 CD80-His ELISA

Example QC data shown. See individual product pages and datasheets for target-specific QC results.

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Available formats

Soluble CD80 ectodomain dimer CSPâ„¢ (Conigen Soluble Protein)

  • Human
  • Mouse
  • Cynomolgus / Rhesus macaque

Tag options

  • hFc
  • His
  • FLAG-His

Available CD80 Products

ProductTagSpeciesProduct CodeDetailshf:tax:product_taghf:tax:pa_species
CD80HisHumanCSP-24033Show detailshishuman
CD80Flag-HisHumanCSP-24033-02Show detailsflag-hishuman
CD80hFcHumanCSP-24033-04Show detailshfchuman
CD80Flag-HisMouseCSP-25187-02Show detailsflag-hismouse
CD80hFcMouseCSP-25187-04Show detailshfcmouse
CD80HisRhesus MacaqueCSP-25293-01Show detailshisrhesus-macaque

Applications

  • Antibody discovery and characterization (screening and specificity testing for CD80-targeting antibodies)
  • Immune checkpoint / co-stimulation interaction studies (ELISA workflows for CD80–CTLA-4 and CD80–CD28 receptor/ligand binding, including competition/blocked-binding assay formats)
  • Binding kinetics and affinity studies (ELISA, SPR and BLI assays for CD80 interactions with antibodies, CTLA-4, and CD28)
  • Animal immunization (RUO) (as an immunogen to support generation of CD80-targeting antibodies)

Quality Control and Specifications

Quality Control Testing

  • Purity assessment (greater than 90% dimer formed as determined by SDS-PAGE under reducing and non-reducing conditions)
  • Bioactivity testing (binding activity confirmed in ELISA and SPR/BLI assays for CD80 interactions with CTLA-4, CD28, and CD80-specific antibodies)
  • Lot-specific documentation provided in downloadable product datasheets

Product Specifications

  • Format: extracellular domain cis-dimers of CD80
  • Expression host: HEK293T (mammalian)
  • Construct: soluble extracellular domain (ECD) dimer fused with a proprietary dimer motif to mimic the native conformation
  • Formulation: 0.22 μm filtered PBS, pH 7.4
  • Storage & shipping: shipped frozen on dry ice; store at −80°C

About Conigen Soluble Proteins

Engineered dimeric proteins with quaternary structures

  • Enhances receptor/ligand binding
  • Increase functional activity
  • Improve binding avidity for better detection sensitivity

Learn more about the CSPâ„¢ (Conigen Soluble Protien) Platform

FAQs

Recombinant CD80 is commonly used in immunology and immuno-oncology research to study T-cell activation, costimulatory signaling, and immune checkpoint pathways. Common applications include:

  • Antibody and therapeutic development
  • Immune checkpoint and tolerance research
  • T-cell activation and costimulation studies

Our CD80 is provided as a dimer and engineered to replicate the native, cell-surface conformation of CD80 more closely than the monomeric ectodomain.

In practice, our dimeric CD80 provides:

  • Higher binding potency
  • Improved assay sensitivity 
  • Wider assay windows

Yes, our recombinant CD80 has been validated for use in binding assays, including ELISA-based CD80-specific antibody binding assays and CTLA-4 and CD28 ligand binding assays.

CD80 is supplied in PBS and is shipped on dry ice. Upon receipt, it should be stored at – 80°C. For optimal stability we recommend aliquoting the protein into smaller volumes before freezing to avoid repeated freeze-thaw cycles.

For short term use, the protein may be stored at 4°C for a limited period. For long-term storage, keep the protein at -80°C for up to 12 months. Avoid vigorous mixing; gently invert or pipette to mix if needed.

CD80 is available from Conigen in the human, mouse and rhesus macaque/cyno forms with His, His-Flag, and Fc tags. 

Additional tags such as Avi, HA, or Myc or formats such as biotinylation can be provided through custom expression to match your assay or conjugation needs.

Yes. CD80 proteins can be customized through Conigen’s CSP â„¢ platform. For custom CD80 formats (alternate tags, variants, or assay-specific constructs), email [email protected] or submit a request through our Custom Project form.

Lead time varies by format and scale. Catalog CD80 can be ordered directly and will ship within 2-3 business days of sales order receipt. 

Custom CD80 and larger orders will follow a defined project workflow, but will generally be shipped 4-6 weeks following request. Contact us for a precise delivery estimate.

Need help selecting the right CD80 format for CD28/CTLA-4 assays?

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