Bioactive, Human IL4Ra Protein Dimer, His Tag
| Product Code | CSP-25219-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-4Rα-specific antibody and interleukin 4 (IL-4) ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IL-4Rα-specific antibody and IL-4 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | M26-H232 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human IL-4Rα protein dimer, His-tag (CSP-25219-01) at 2 μg/mL (100 μL/well) can bind anti-human IL-4Rα monoclonal antibody with half maximal effective concentration (EC50) range of 6-24 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human IL-4Rα protein dimer, His-tag (CSP-25219-01) at 2 μg/mL (100 μL/well) can bind human IL-4 with half maximal effective concentration (EC50) range of 3.9-15.6 ng/mL (QC tested).
SDS-PAGE
NR: IL-4Rα dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human interleukin 4 receptor alpha (IL-4Rα) is a Type I transmembrane and a Type I cytokine receptor. The recombinant IL-4Rα protein dimer (CSP-25219-01) is a cis-homodimer (cis-dimer) and contains a IL-4Rα extracellular domain (UniProt# P24394, amino acids Met26-His232) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human IL-4Rα protein dimer is bioactive and can bind to interleukin 4 (IL-4). It also binds IL-4Rα-specific antibodies. This IL-4Rα protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL4Ra
UniProt #: P24394
Predicted Molecular Weight: 64 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 120 and 190 kDa on SDS PAGE.
Protein Construct: IL-4Rα dimer protein contains a IL-4Rα extracellular domain (UniProt# P24394) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Human interleukin 4 receptor alpha (IL-4Rα) is a Type I transmembrane and a Type I cytokine receptor. IL-4Rα is also known as cluster of differentiation 124 (CD124). IL-4Rα is a key component in interleukin 4 (IL-4) and IL-13 cytokine signaling involved in immune regulation, particularly in Th2 immune responses, allergy, and asthma. IL-4Rα contains an extracellular domain with an overall L shape organized in two covalently linked domains: an h-type immunoglobulin fold (D1) and a standard fibronectin type III (FN III)–like topology (D2). IL-4Rα can bind IL-4 and IL-13 and allergic inflammation is largely driven by IL-4 and IL-13 signaling through IL-4Rα, making IL-4Rα a promising therapeutic target for allergic diseases.
Alternate Names: IL4R, cluster of differentiation 124, CD124, IL-4RA, IL4RA, Interleukin-4 receptor