Mouse IL-7Rα/TSLP R Protein Heterodimer, His and Strep Tag
| Product Code | CSP-25242-A1B6 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-7Rα and TSLP R-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for IL-7Rα and TSLP R-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | AA: E21-D239 ; BA: A20-P232 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human IL-7Rα/TSLP R protein heterodimer, His and Strep-tag (CSP-25242-A1B6) at 2 μg/mL (100 μL/well) can bind anti-human TSLP R monoclonal antibody with half maximal effective concentration (EC50) range of 14.4-57.5 ng/mL (QC tested).
Bioactivity – Antibody Binding
Immobilized human IL-7Rα/TSLP R protein heterodimer, His and Strep-tag (CSP-25242-A1B6) at 2 μg/mL (100 μL/well) can bind anti-human IL-7Rα polyclonal antibody with half maximal effective concentration (EC50) range of 4.9-19.5 ng/mL (QC tested).
SDS-PAGE
NR: IL-7Rα/TSLP R heterodimer under non-reduced condition
R: IL-7Rα/TSLP R heterodimer under reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Interleukin-7 receptor subunit alpha (IL-7Rα) and thymic stromal lymphopoietin receptor (TSLP R) compose a protein heterodimeric receptor complex. The recombinant IL-7Rα/TSLP R protein (CSP-25242-A1B6) is a heterodimer and contains an IL-7Rα extracellular domain (UniProt# P16872, amino acids Glu21-Asp239) and a TSLP R extracellular domain (UniProt# Q8CII9, amino acids Ala20-Pro232) fused with a proprietary cis-dimer motif followed by a His tag at the IL-7Rα C-terminus and a Strep tag at the TSLP R C-terminus. This heterodimeric protein is expressed in HEK293T cells. The recombinant mouse IL-7Rα/TSLP R heterodimer binds IL-7Rα and TSLP R-specific antibodies. This IL-7Rα/TSLP R heterodimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL-7Ra / TSLP R
UniProt #: AA: P16872 ; BA: Q8CII9
Predicted Molecular Weight: 63 kDa
SDS PAGE Molecular Weight: The migration range of the protein heterodimer with glycosylation under non-reducing condition is ~120 kDa and under reducing condition between 40 kDa for TSLP R chain and 50 kDa for IL-7Rα chain on SDS PAGE.
Protein Construct: IL-7Rα/TSLP R heterodimer protein contains an IL-7Rα extracellular domain (UniProt# P16872, amino acids Glu21-Asp239) and a TSLP R extracellular domain (UniProt# Q8CII9, amino acids Ala20-Pro232) fused with a proprietary dimer motif followed by a His tag at the IL-7Rα C-terminus and a Strep tag at the TSLP R C-terminus.
Background
Interleukin-7 receptor subunit alpha (IL-7Rα) and thymic stromal lymphopoietin receptor (TSLP R) compose a protein heterodimeric receptor complex that binds the pro-inflammatory cytokine TSLP. IL-7Rα, also known as Cluster of Differentiation 127 (CD127), is a transmembrane receptor belonging to the cytokine receptor homology class 1 (CRH1) family with an extracellular domain consisting of two fibronectin type III (FNIII) domains (D1 and D2). TSLP R, also known as Cytokine receptor-like factor 2 (CRLF2), contains an extracellular domain consisting of a single CRH module composed of two tandem fibronectin type III (FnIII) domains (D1 and D2). The IL-7Rα/TSLP R heterodimer is central to the development of widespread allergic diseases, including asthma and atopic dermatitis, making it an attractive therapeutic target. While structurally and functionally similar to human IL-7Rα/TSLP R heterodimer, mouse IL-7Rα/TSLP R heterodimer is a species-specific tool essential for preclinical studies, basic research, and translational research in cancer immunotherapy.
Alternate Names: Cluster of Differentiation 127, CD127, IL7R, CD127, CDW127, IL-7R-alpha, IL7RA, ILRA, Interleukin-7 receptor-α, interleukin 7 receptor, IL7R-α, Cytokine receptor-like factor 2, CRLF2, CRL2, CRLF2Y, TSLPR