Bioactive, Recombinant Rhesus macaque TIGIT Protein Dimer, His Tag
| Product Code | CSP-25298-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for TIGIT-specific antibody binding assays. SPR for TIGIT and PVR ligand protein binding assays. |
| Suggested Applications | BLI and ELISA for TIGIT and PVR protein binding assays and BLI for TIGIT-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | M22-P142 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized Rhesus macaque TIGIT protein dimer, His Tag (CSP-25298-01) at 2 μg/mL (100 μL/well) can bind anti-human TIGIT monoclonal antibody with half maximal effective concentration (EC50) range of 0.2-0.7 μg/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: TIGIT dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Rhesus macaque TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 and VSTM3. The recombinant TIGIT protein dimer (CSP-25298-01) is a cis-homodimer (cis-dimer) and contains a TIGIT extracellular domain (UniProt# A0A5F8AKQ5, amino acids Met22-Pro142) fused with a proprietary dimer motif followed by a His-tag at the C-terminus. This protein is expressed in HEK293T cells. The TIGIT-His dimer protein is bioactive and can bind to PVR and TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.
Protein Name: TIGIT
UniProt #: A0A5F8AKQ5
Predicted Molecular Weight: 43 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing conditions is between 60 and 85 kDa on SDS PAGE.
Protein Construct: TIGIT protein dimer contains a TIGIT extracellular domain (UniProt# A0A5F8AKQ5) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9) and VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a Type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT is a pivotal immune checkpoint receptor that fine-tunes T-cell and NK-cell responses. Its dual role in promoting tolerance (via Tregs) and suppressing anti-tumor immunity makes it a compelling therapeutic target. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT. While structurally and functionally similar to human TIGIT, Rhesus macaque TIGIT is a species-specific tool essential for translational research in cancer immunotherapy.
Alternate Names: VSIG9, VSTM3