Human Siglec 3 / CD33 Protein Dimer, His Tag
| Product Code | CSP-25200-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for Siglec 3-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for Siglec 3-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | D18-H259 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human Siglec 3 protein dimer, His Tag (CSP-25200-01) at 2 μg/mL (100 μL/well) can bind anti-human Siglec 3 monoclonal antibody with half maximal effective concentration (EC50) range of 0.23-0.91 μg/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: Siglec 3 dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human Sialic acid-binding Ig-like lectin 3 (Siglec 3) is a Type I transmembrane protein and a member of the Siglec family and immunoglobulin superfamily. The recombinant Siglec 3 dimer protein (CSP-25200-01) is a cis-homodimer (cis-dimer) and contains a Siglec 3 extracellular domain (UniProt# P20138, amino acids Asp18-His259) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Siglec 3 protein dimer can potently bind Siglec 3-specific antibodies. This Siglec 3 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CD33
UniProt #: P20138
Predicted Molecular Weight: 70 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is ~120 kDa on SDS PAGE.
Protein Construct: Siglec 3 dimer protein contains a Siglec 3 extracellular domain (UniProt# P20138) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Human Sialic acid-binding Ig-like lectin 3 (Siglec 3, Siglec-3) is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec 3 is also known as Cluster of Differentiation (CD33) and p67. It’s part of the immunoglobulin superfamily, and a multifunctional immune regulator in the innate immune system. Siglec 3 is a Type I transmembrane protein and can form homodimers on the cell surface. It contains an extracellular domain with an N-terminal V-type immunoglobulin domain (Ig domain) and a C2-type Ig domains followed by a transmembrane domain and cytoplasmic signaling domain consisting of the immunoreceptor tyrosine-based inhibitory motif (ITIM). Siglec 3/CD33 is expressed on myeloid progenitors and monocytes. It binds sialylated glycans on cell surfaces or secreted proteins, modulating cell-cell interactions and immune responses. The overexpression of Siglec 3 is linked with the development of Alzheimer’s disease and is an emerging target for therapeutics.
Alternate Names: cluster of differentiation 33, CD33, p67