Bioactive, Recombinant Human IL-6Rα/gp130 Protein Heterodimer, His and Strep Tag
| Product Code | CSP-25249-A1B6 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-6Rα and gp130-specific antibodies and ELISA and SPR for interleukin 6 (IL-6) ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IL-6Rα and gp130-specific antibodies and BLI for IL-6 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | L20-P365 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human IL-6Rα/gp130 protein heterodimer, His and Strep-tag (CSP-25249-A1B6) at 2 μg/mL (100 μL/well) can bind anti-human gp130 monoclonal antibody with half maximal effective concentration (EC50) range of 20.3-81.2 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human IL-6 at 2 μg/mL (100 μL/well) can bind human IL-6Rα/gp130 protein heterodimer, His and Strep-tag (CSP-25249-A1B6) with half maximal effective concentration (EC50) range of 36.3-145.1 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: IL-6Rα/gp130 heterodimer under non-reduced condition
R: IL-6Rα/gp130 heterodimer under reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human interleukin 6 receptor is a heterodimer consisting of IL-6Rα and gp130 (IL-6Rβ). The recombinant IL-6R protein (CSP-25249-A1B6) is a heterodimer and contains an IL-6Rα extracellular domain (UniProt# P08887, amino acids Leu20-Pro365) and gp130 extracellular domain (UniProt# P40189, amino acids Glu23-Glu619) fused with a proprietary dimer motif followed by a His tag at the IL-6Rα C-terminus and a Strep tag at the gp130 C-terminus. This heterodimeric protein is expressed in HEK293T cells. The recombinant human IL-6R heterodimer protein is bioactive and can bind to interleukin 6 (IL-6). It also binds IL-6Rα and gp130-specific antibodies. This IL-6R heterodimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL6R_IL6ST
UniProt #: AA: P08887
Predicted Molecular Weight: 122 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is ~190 kDa and under reducing condition ~120 for IL-6Rα chain and ~85 kDa for gp130 (IL-6Rβ) chain on SDS PAGE.
Protein Construct: IL-6R heterodimer protein contains an IL-6Rα extracellular domain (UniProt# P08887, amino acids Leu20-Pro365) and gp130 extracellular domain (UniProt# P40189, amino acids Glu23-Glu619) fused with a proprietary dimer motif followed by a His tag at the IL-6Rα C-terminus and a Strep tag at the gp130 C-terminus.
Background
Human interleukin 6 receptor (IL-6R) is a heterodimer consisting of IL-6Rα and gp130 (IL-6Rβ). Both IL-6Rα and gp130 are Type I transmembrane proteins. IL-6Rα is Type I cytokine receptor and gp130 is a member of the class of tall cytokine receptors. IL-6Rα contains an extracellular domain with an Ig-like domain, cytokine binding module (CBM) domains, and a long flexible stalk region followed by a transmembrane domain and intracellular domains. The extracellular domain of gp130 includes an N-terminal immunoglobulin-like (Ig-like) domain (D1), a cytokine-binding homology region (CHR, D2D3), and three membrane-proximal fibronectin type III domains (FNIII, D4 to D6). IL-6Rα binding to its ligand interleukin 6 (IL-6) results in homodimerization and subsequent association with gp130 homodimer resulting in higher order complexes. The interaction between IL-6 cytokine and IL-6R is crucial for immune responses, inflammation, and hematopoiesis. Dysregulation of IL-6R is implicated in many cancers and autoimmune diseases, therefore, a recombinant protein mimicking the IL-6R heterodimer conformation can be critical for immunology research and therapeutic discovery.
Alternate Names: Cluster of Differentiation 126, CD126, IL6R, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80, Interleukin 6 Cytokine Family Signal Transducer, IL6ST, Cluster of Differentiation 130, CD130, CDW130, GP130, Interleukin-6 receptor subunit beta, IL6Rb, IL-6RB, IL-6R beta, IL6β, interleukin 6 signal transducer