Bioactive, Recombinant Rhesus macaque PVR Protein Dimer, His Tag
| Product Code | CSP-25291-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for CD19-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for CD19-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | P20-K292 |
For Research Use Only (RUO)
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Antibody Binding
Immobilized Rhesus macaque PVR protein dimer, His Tag (CSP-25297-01) at 2 μg/mL (100 μL/well) can bind anti-non-human primate PVR monoclonal antibody with half maximal effective concentration (EC50) range of 10.5-42 ng/mL (QC tested).
Ligand Binding
Immobilized Rhesus macaque TIGIT at 2 μg/mL (100 μL/well) can bind Rhesus macaque PVR protein dimer, His Tag (CSP-25297-01) with half maximal effective concentration (EC50) range of 10.5-42 ng/mL (QC tested).
SDS-PAGE
NR: PVR dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Poliovirus Receptor (PVR) is a Type I transmembrane glycoprotein that belongs to the Nectin/Nectin-like family. This recombinant Rhesus macaque PVR protein dimer (CSP-25297-01) is a cis-homodimer (cis-dimer) and contains the PVR extracellular domain (UniProt# Q0MSE6, amino acids Trp21-Asn343) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This protein is expressed in HEK293T cells. This Rhesus macaque PVR dimer protein is bioactive and can bind to Rhesus macaque TIGIT. It also binds PVR-specific antibodies. This Rhesus macaque PVR protein dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.
Protein Name: PVR
UniProt #: Q0MSE6
Predicted Molecular Weight: 86 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing conditions is between 120 and 190 kDa on SDS PAGE.
Protein Construct: PVR protein dimer contains a PVR extracellular domain (Uniprot# Q0MSE6) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Poliovirus Receptor (PVR) is a Type I transmembrane glycoprotein that belongs to the Nectin/Nectin-like family. PVR is also known as cluster of differentiation 155 (CD155), HVED, NECL5, Necl-5, PVS, TAGE4, and FLJ25946. PVR protein consists of 3 extracellular immunoglobulin-like (Ig-like) domains (D1-D3), one transmembrane region, and a C-terminal cytoplasmic domain. PVR is widely expressed on various cell types and often overexpressed on cancer cells. Upregulation of PVR in several types of human cancers is associated with a poor prognosis. Although PVR itself is not an immune checkpoint, it’s a ligand for checkpoint receptors like TIGIT (T-cell immunoreceptor with Ig and ITIM domains), CD226 (DNAM-1), and CD96. The interactions between PVR and its receptors on immune cells modulate immune responses, such as T cells and natural killer (NK) cells. This makes PVR a critical player in immune regulation and a promising target in checkpoint cancer therapies. While structurally and functionally similar to human PVR homodimer, Rhesus macaque PVR homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.
Alternate Names: PVR, HVED, NECL5, Necl-5, PVS, FLJ25946, and TAGE4