Recombinant Mouse IL-6Rα Protein Dimer, His Tag
| Product Code | CSP-25224-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-6Rα-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for IL-6Rα-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | L20-P364 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized mouse IL-6Rα protein dimer, His-tag (CSP-25224-01) at 2 μg/mL (100 μL/well) can bind anti-mouse IL-6Rα polyclonal antibody with half maximal effective concentration (EC50) range of 5.4-21.5 ng/mL (QC tested).
SDS-PAGE
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Interleukin 6 receptor alpha (IL-6Rα) is a Type I transmembrane protein and Type I cytokine receptor. The recombinant IL-6Rα dimer protein (CSP-25224-01) is a cis-homodimer (cis-dimer) and contains an IL-6Rα extracellular domain (UniProt# P22272, amino acids Leu20-Pro364) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse IL-6Rα dimer protein is bioactive and can bind to interleukin 6 (IL-6). It also binds IL-6Rα-specific antibodies. This IL-6Rα dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL6R
UniProt #: P22272
Predicted Molecular Weight: 92 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is between 120 and 190 kDa on SDS PAGE.
Protein Construct: IL-6Rα protein dimer contains the IL-6Rα extracellular domain (UniProt# P22272) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Interleukin 6 receptor alpha (IL-6Rα) is a Type I transmembrane protein and Type I cytokine receptor and plays a crucial role in immune response, inflammation, and hematopoiesis. IL-6Rα is also known as Cluster of Differentiation 126 (CD126), IL6R, CD126, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, and gp80. IL-6Rα contains an extracellular domain with an Ig-like domain, cytokine binding module (CBM) domains, and a long flexible stalk region followed by a transmembrane domain and intracellular domains. IL-6Rα binding to its ligand interleukin 6 (IL-6) results in homodimerization and subsequent association with IL-6Rβ (gp130) dimer resulting in higher order complexes. IL-6Rα is involved in multiple disease processes due to its central role in IL-6 signaling. Dysregulation of IL-6Rα is implicated in many cancers and autoimmune diseases. While structurally and functionally similar to mouse IL-6Rα, mouse IL-6Rα is a species-specific tool essential for preclinical studies, basic research and translational research in cancer immunotherapy.
Alternate Names: Cluster of Differentiation 126, CD126, IL6R, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80, Interleukin-6 receptor, interleukin 6 receptor